Liquid Biopsy Sequencing (LB-Seq)

Our laboratory and computational methods for generation and analysis of DNA sequence from circulating tumour DNA.

We have protocols to collect blood for cell-free DNA profiling, extract cell-free DNA from 1-25 mL of blood plasma, construct DNA sequencing libraries, perform hybrid capture using a variety of stock and custom panels, and sequence to >20,000X coverage at the Princess Margaret Genomics Centre (www.pmgenomics.ca). This protocol is described in our paper (Link to Kis et al publication) and the software is freely available here: https://github.com/pughlab/LB-Seq